Multiplex qPCR: Criteria for Success
Current best practices for gene expression analysis by qPCR recommend the use of multiple normalizers and, potentially, a second gene of interest (GOI) assay. For samples with limited nucleic acid content, such an experiment may only be achieved by performing multiplexed qPCR assays. Even when working with abundant nucleic acids, multiplexing can save time and money. Achieving successful multiplex assays depends upon several criteria, including using robust assays, correct reagent and target concentrations, and appropriate cycling parameters. Using data from several assays, we demonstrate how PrimeTime® qPCR Assays simplify a 4-plex assay system, and discuss factors that influence the success of using these assays in a multiplex format. We also compare the use of master mixes assembled de novo to commercially available reagents and discuss their effects on multiplex assays. About the Speaker: Scott Rose, PhD, is the Director of Molecular Genetics at Integrated DNA Technologies. He leads a group of scientists involved in numerous projects and research collaborations in areas such as qPCR, RNAi, antisense, RNase H2--based detection, and synthetic biology. The focus of his postdoctoral research was transcription-based gene regulation using the pancreas as a model system. For his PhD in Biochemistry, he studied RNA processing, concentrating on RNA splicing and polyadenylation.From:idtdnabioViews:5 0ratingsTime:01:09:12More inScience Technology

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Education of the Heart and Mind: Santa Ono at TEDxEmory 2012
Santa J. Ono is a biomedical scientist and university administrator. He currently serves as Senior Vice President for Academic Affairs and University Provost at the University of Cincinnati. Prior to his move to UC, Santa served as Vice Provost for Academic Initiatives and Deputy to the Provost and then Senior Vice Provost for Undergraduate Academic Affairs at Emory University. He was educated at the University of Chicago, McGill, and Harvard, and was a Helen Hay Whitney Fellow in Biochemistry Molecular Biology at Harvard. He has taught at Johns Hopkins, Harvard, University College London, and Emory. In this talk, Ono examines the path to Global Citizens through American Higher Education. AboutTEDx, x = independently organized event In the spirit of ideas worth spreading, TEDx is a program of local, self-organized events that bring people together to share a TED-like experience. At a TEDx event, TEDTalks video and live speakers combine to spark deep discussion and connection in a small group. These local, self-organized events are branded TEDx, where x = independently organized TED event. The TED Conference provides general guidance for the TEDx program, but individual TEDx events are self-organized.* (*Subject to certain rules and regulations)From:TEDxTalksViews:3 2ratingsTime:20:30More inEducation

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And it was all yellow....
A video I created along with my group for our Clinical Biochemistry Course in Medical Laboratory Science on a case study given to us. Lab values and patient history were given, and we had to make a diagnosis. The video is loosely based on the TV show House MD.From:tino7017Views:0 0ratingsTime:08:32More inEducation

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And it was all yellow.... - Video

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Dato #39; Dr. Halo-N #39; (MALAYSIA) receives Gusi Peace Prize 2012
Dato #39; Dr. Haji Ahmad Laksamana Bin of Malaysia Omar receives Gusi Peace Prize 2012 For his contributions as the first Al-Quranic Scientist of the World in the Field of Research(Biochemistry). By interpreting the meaning of the Noble Quran, he made a significant breakthrough in the area of Biochemistry by successfully establishing five formula and theories to the field of natural products.From:gusipeaceprizeViews:2 0ratingsTime:03:42More inPeople Blogs

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Nucleosome Rock Star Scientist Karolin Luger at Colorado State University
Karolin Luger, University Distinguished Professor and Investigator for the Howard Hughes Medical Institute at Colorado State University, can add another award to her list of accomplishments: National Lecturer for the Biophysical Society, which is one of its most prestigious honors. Her research focuses on the structure, function and assembly of the nucleosome, using a wide variety of biophysical approaches. Her work is now cited in nearly every modern textbook of biochemistry and molecular biology. For more videos about CSU, check out Colorado State University #39;s YouTube channel at http://www.youtube.comFrom:ColoradoStateUnivViews:2 0ratingsTime:02:50More inScience Technology

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Allosteric regulation/ Sequential Allostery / Allosteric model / allosteric regulation of enzyme
In biochemistry, allosteric regulation is the regulation of an enzyme or other protein by binding an effector molecule at the protein #39;s allosteric site (that is, a site other than the protein #39;s active site)From:Biotech ReviewViews:0 0ratingsTime:00:27More inScience Technology

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