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Scientists Work Towards a CRISPR-based COVID-19 Therapy – Technology Networks

A team of scientists from Stanford University is working with researchers at theMolecular Foundry, a nanoscience user facility located at the Department of Energy's Lawrence Berkeley National Laboratory (Berkeley Lab), to develop a gene-targeting, antiviral agent against COVID-19.

Last year, Stanley Qi, an assistant professor in the departments of bioengineering, and chemical and systems biology at Stanford University and his team had begun working on a technique called PAC-MAN - or Prophylactic Antiviral CRISPR in human cells - that uses the gene-editing tool CRISPR to fight influenza.

But that all changed in January, when news of the COVID-19 pandemic emerged. Qi and his team were suddenly confronted with a mysterious new virus for which no one had a clear solution. "So we thought, 'Why don't we try using our PAC-MAN technology to fight it?'" said Qi.

Since late March, Qi and his team have been collaborating with a group led byMichael Connolly, a principal scientific engineering associate in the Biological Nanostructures Facility at Berkeley Lab's Molecular Foundry, to develop a system that delivers PAC-MAN into the cells of a patient.

Like all CRISPR systems, PAC-MAN is composed of an enzyme - in this case, the virus-killing enzyme Cas13 - and a strand of guide RNA, which commands Cas13 to destroy specific nucleotide sequences in the coronavirus's genome. By scrambling the virus's genetic code, PAC-MAN could neutralize the coronavirus and stop it from replicating inside cells.

It's all in the delivery

Qi said that the key challenge to translating PAC-MAN from a molecular tool into an anti-COVID-19 therapy is finding an effective way to deliver it into lung cells. When SARS-CoV-2, the coronavirus that causes COVID-19, invades the lungs, the air sacs in an infected person can become inflamed and fill with fluid, hijacking a patient's ability to breathe.

"But my lab doesn't work on delivery methods," he said. So on March 14, they published a preprint of their paper, and even tweeted, in the hopes of catching the eye of a potential collaborator with expertise in cellular delivery techniques.

Soon after, they learned of Connolly's work on synthetic molecules called lipitoids at the Molecular Foundry.

Lipitoids are a type of synthetic peptide mimic known as a "peptoid" first discovered 20 years ago by Connolly's mentor Ron Zuckermann. In the decades since, Connolly and Zuckermann have worked to develop peptoid delivery molecules such as lipitoids. And in collaboration with Molecular Foundry users, they have demonstrated lipitoids' effectiveness in the delivery ofDNAandRNAto a wide variety of cell lines.

Today, researchers studying lipitoids for potentialtherapeutic applicationshave shown that these materials are nontoxic to the body and can deliver nucleotides by encapsulating them in tiny nanoparticles just one billionth of a meter wide - the size of a virus.

Now Qi hopes to add his CRISPR-based COVID-19 therapy to the Molecular Foundry's growing body of lipitoid delivery systems.

In late April, the Stanford researchers tested a type of lipitoid - Lipitoid 1 - that self-assembles with DNA and RNA into PAC-MAN carriers in a sample of human epithelial lung cells.

According to Qi, the lipitoids performed very well. When packaged with coronavirus-targeting PAC-MAN, the system reduced the amount of synthetic SARS-CoV-2 in solution by more than 90%. "Berkeley Lab's Molecular Foundry has provided us with a molecular treasure that transformed our research," he said.

The team next plans to test the PAC-MAN/lipitoid system in an animal model against a live SARS-CoV-2 virus. They will be joined by collaborators at New York University and Karolinska Institute in Stockholm, Sweden.

If successful, they hope to continue working with Connolly and his team to further develop PAC-MAN/lipitoid therapies for SARS-CoV-2 and other coronaviruses, and to explore scaling up their experiments for preclinical tests.

"An effective lipitoid delivery, coupled with CRISPR targeting, could enable a very powerful strategy for fighting viral disease not only against COVID-19 but possibly against newly viral strains with pandemic potential," said Connolly.

"Everyone has been working around the clock trying to come up with new solutions," added Qi, whose preprint paper was recently peer-reviewed and published in the journalCell. "It's very rewarding to combine expertise and test new ideas across institutions in these difficult times."

Reference: Abbott et al. (2020). Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Cell. DOI: https://doi.org/10.1016/j.cell.2020.04.020.

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source.

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Scientists Just Created Artificial Red Blood Cells That May Be Even Better Than The Real Thing – ScienceAlert

Thanks to rapid advances in technology, prosthetic limbs and artificial organs are pushing past the limits of what nature provides us. It's now blood's turn for a boost, with researchers finding a new way to mimic the tissue that carries oxygen.

By combining biological material with lab-grown polymers, an international team of bioengineers has developed what could be thought of as a Terminator red cell - one more than capable of matching the talents of what's already in our veins.

Not only can this microscopic cyborg squeeze through the nooks and crannies of a vascular system with its usual haemoglobin, it can be modified to deliver tumour-killing medications, carry biosensors, and even be studded with tiny magnets for the ultimate in remote guidance.

If you're going to attempt to reconfigure any cell in the human body, you really can't go past the red blood cell (RBC). One of the few cells that lack a nucleus, the red cell has a relative simplicity that makes it an attractive target for engineers to build upon.

What's more, our dependence on huge amounts of clean, freshly donated products to replace blood lost through trauma puts a high demand on finding a suitable substitute.

Hence, there are several synthetic RBCs already in development. Many rely on scavenging key materials such as haemoglobin from human or animal donors and repackaging them into benign particles that are unlikely to trigger an immune response.

Some of the avenues being explored are a little more adventurous, going as far as creating sound-powered particles that can carry toxic species of highly reactive oxygen through the vascular system to assassinate cancerous tissues.

"Inspired by the above pioneering studies wherein synthetic constructs were created that achieved one or several key features of native RBCs, we endeavoured to create a modular rebuilt RBC (RRBC) mimic that possessed the complete combined features of native RBCs," the researchers write in their report.

That means their bionic mimics had to be an appropriate size, shape, and flexibility to make it through the body's narrowest vessels; remain intact long enough to be useful; and still carry a suitable amount of oxygen.

Taking it even further, the team figured they'd make their tiny units modular, allowing them to swap in and out various features that helped the cells carry drugs or hone in on a destination.

To achieve their goal, they started by coating donated blood cells in a layer of silica, which was then painted with polymers of different charges.

Once the silica and cell guts were scraped away, the remaining polymer membrane could itself be covered in a skin made from red blood cells.

The result is an empty biconcave shell that can be packed with whatever biochemical machinery your heart desires, while still passing for a garden variety, oxygen-hauling red blood cell.

A battery of tests using lab equipment and animals showed the bionic blood cells lived up to expectations. Four weeks after being injected into mice, there were no signs of adverse effects, boding well for the safety of these synthetic cells.

Clearly there's a long way to go before we'll see any therapies based on artificial blood cells. Not only will we need plenty of testing to check if any cargo they carry can be released, the whole manufacturing process needs to be shown to be scalable.

But with so much research focussed on building a better blood cell, there's little doubt we'll be seeing more in this field soon.

Turning our body's own cells into tiny murderous robots to attack wayward tissues and infections seems to be a popular strategy for bioengineers. Time will tell if it's a winning formula.

Be assured, we'll be back if it is.

This research was published in ACS Nano.

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Global Microbial Identification System Market 2020 Research Report and Forecast to 2026 – Cole of Duty

The global Microbial Identification System market focuses on encompassing major statistical evidence for the Microbial Identification System industry as it offers our readers a value addition on guiding them in encountering the obstacles surrounding the market. A comprehensive addition of several factors such as global distribution, manufacturers, market size, and market factors that affect the global contributions are reported in the study. In addition the Microbial Identification System study also shifts its attention with an in-depth competitive landscape, defined growth opportunities, market share coupled with product type and applications, key companies responsible for the production, and utilized strategies are also marked.

This intelligence and 2026 forecasts Microbial Identification System industry report further exhibits a pattern of analyzing previous data sources gathered from reliable sources and sets a precedented growth trajectory for the Microbial Identification System market. The report also focuses on a comprehensive market revenue streams along with growth patterns, analytics focused on market trends, and the overall volume of the market.

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The study covers the following key players:Merck MilliporeAdvanced Instruments IncBDHangzhou Tailin Bioengineering EquipmentsPZ Cormay

Moreover, the Microbial Identification System report describes the market division based on various parameters and attributes that are based on geographical distribution, product types, applications, etc. The market segmentation clarifies further regional distribution for the Microbial Identification System market, business trends, potential revenue sources, and upcoming market opportunities.

Market segment by type, the Microbial Identification System market can be split into,Protein Based Identification Method

Market segment by applications, the Microbial Identification System market can be split into,HospitalLaboratory

The Microbial Identification System market study further highlights the segmentation of the Microbial Identification System industry on a global distribution. The report focuses on regions of North America, Europe, Asia, and the Rest of the World in terms of developing business trends, preferred market channels, investment feasibility, long term investments, and environmental analysis. The Microbial Identification System report also calls attention to investigate product capacity, product price, profit streams, supply to demand ratio, production and market growth rate, and a projected growth forecast.

In addition, the Microbial Identification System market study also covers several factors such as market status, key market trends, growth forecast, and growth opportunities. Furthermore, we analyze the challenges faced by the Microbial Identification System market in terms of global and regional basis. The study also encompasses a number of opportunities and emerging trends which are considered by considering their impact on the global scale in acquiring a majority of the market share.

The study encompasses a variety of analytical resources such as SWOT analysis and Porters Five Forces analysis coupled with primary and secondary research methodologies. It covers all the bases surrounding the Microbial Identification System industry as it explores the competitive nature of the market complete with a regional analysis.

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Some Point of Table of Content:

Chapter One: Microbial Identification System Market Overview

Chapter Two: Global Microbial Identification System Market Landscape by Player

Chapter Three: Players Profiles

Chapter Four: Global Microbial Identification System Production, Revenue (Value), Price Trend by Type

Chapter Five: Global Microbial Identification System Market Analysis by Application

Chapter Six: Global Microbial Identification System Production, Consumption, Export, Import by Region (2014-2019)

Chapter Seven: Global Microbial Identification System Production, Revenue (Value) by Region (2014-2019)

Chapter Eight: Microbial Identification System Manufacturing Analysis

Chapter Nine: Industrial Chain, Sourcing Strategy and Downstream Buyers

Chapter Ten: Market Dynamics

Chapter Eleven: Global Microbial Identification System Market Forecast (2019-2026)

Chapter Twelve: Research Findings and Conclusion

Chapter Thirteen: Appendix continued

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List of tablesList of Tables and FiguresFigure Microbial Identification System Product PictureTable Global Microbial Identification System Production and CAGR (%) Comparison by TypeTable Profile of Protein Based Identification MethodTable Microbial Identification System Consumption (Sales) Comparison by Application (2014-2026)Table Profile of HospitalTable Profile of LaboratoryFigure Global Microbial Identification System Market Size (Value) and CAGR (%) (2014-2026)Figure United States Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Europe Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Germany Microbial Identification System Revenue and Growth Rate (2014-2026)Figure UK Microbial Identification System Revenue and Growth Rate (2014-2026)Figure France Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Italy Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Spain Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Russia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Poland Microbial Identification System Revenue and Growth Rate (2014-2026)Figure China Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Japan Microbial Identification System Revenue and Growth Rate (2014-2026)Figure India Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Southeast Asia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Malaysia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Singapore Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Philippines Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Indonesia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Thailand Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Vietnam Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Central and South America Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Brazil Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Mexico Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Colombia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Middle East and Africa Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Saudi Arabia Microbial Identification System Revenue and Growth Rate (2014-2026)Figure United Arab Emirates Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Turkey Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Egypt Microbial Identification System Revenue and Growth Rate (2014-2026)Figure South Africa Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Nigeria Microbial Identification System Revenue and Growth Rate (2014-2026)Figure Global Microbial Identification System Production Status and Outlook (2014-2026)Table Global Microbial Identification System Production by Player (2014-2019)Table Global Microbial Identification System Production Share by Player (2014-2019)Figure Global Microbial Identification System Production Share by Player in 2018Table Microbial Identification System Revenue by Player (2014-2019)Table Microbial Identification System Revenue Market Share by Player (2014-2019)Table Microbial Identification System Price by Player (2014-2019)Table Microbial Identification System Manufacturing Base Distribution and Sales Area by PlayerTable Microbial Identification System Product Type by PlayerTable Mergers & Acquisitions, Expansion PlansTable Merck Millipore ProfileTable Merck Millipore Microbial Identification System Production, Revenue, Price and Gross Margin (2014-2019)Table Advanced Instruments Inc ProfileTable Advanced Instruments Inc Microbial Identification System Production, Revenue, Price and Gross Margin (2014-2019)Table BD ProfileTable BD Microbial Identification System Production, Revenue, Price and Gross Margin (2014-2019)Table Hangzhou Tailin Bioengineering Equipments ProfileTable Hangzhou Tailin Bioengineering Equipments Microbial Identification System Production, Revenue, Price and Gross Margin (2014-2019)Table PZ Cormay ProfileTable PZ Cormay Microbial Identification System Production, Revenue, Price and Gross Margin (2014-2019)Table Global Microbial Identification System Production by Type (2014-2019)Table Global Microbial Identification System Production Market Share by Type (2014-2019)Figure Global Microbial Identification System Production Market Share by Type in 2018Table Global Microbial Identification System Revenue by Type (2014-2019)Table Global Microbial Identification System Revenue Market Share by Type (2014-2019)Figure Global Microbial Identification System Revenue Market Share by Type in 2018Table Microbial Identification System Price by Type (2014-2019)Figure Global Microbial Identification System Production Growth Rate of Protein Based Identification Method (2014-2019)Table Global Microbial Identification System Consumption by Application (2014-2019)Table Global Microbial Identification System Consumption Market Share by Application (2014-2019)Table Global Microbial Identification System Consumption of Hospital (2014-2019)Table Global Microbial Identification System Consumption of Laboratory (2014-2019)Table Global Microbial Identification System Consumption by Region (2014-2019)Table Global Microbial Identification System Consumption Market Share by Region (2014-2019)Table United States Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table Europe Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table China Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table Japan Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table India Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table Southeast Asia Microbial Identification System Production, Consumption, Export, Import (2014-2019)Table Central and South America Microbial Identification System Production, Consumption, Export, Import (2014-2019) continued

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Scientists Have Engineered Human Cells With a Squid-Like Power of Invisibility – ScienceAlert

The opalescent inshore squid has a superpower. Not only can it change the colour of its skin - which many chephalopscan do -it can also turn parts of itself invisible. Now, scientists have used this ability on human cells.

Using special proteins found in the cells of these changeling squids, researchers managed to apply them to human kidney cells. Their findings could help us to better understand various cellular mechanisms in living tissue.

"Our project centres on designing and engineering cellular systems and tissues with controllable properties for transmitting, reflecting and absorbing light," explained biomolecular engineer Atrouli Chatterjee from the University of California (UCI).

A female opalescent inshore squid with her eggs. (Brent Durand/Moment/Getty Images)

Squids aren't the only animals to make use of see-through skin. While gliding lizards (Draco sumatranus) use their skin translucency to draw attention, opalescent inshore squids (Doryteuthis opalescens) use theirs to avoid unwanted attention.

Females of this squid species can turn a white stripe along their backs from opaque white to nearly transparent. They do this using specialised cells called leucophores, which have membrane-bound particles made of reflectin proteins.

Depending on how these proteins are arranged,they can change how light is transmitted or reflected around them. And it's not a random process: Squids can alter the arrangement of these highly refractive proteins within their cells, using an organic chemical called acetylcholine.

To try this trick in human tissue, the research team genetically engineered human kidney cells to produce reflectins, which clumped together as disordered particles in the cell's cytoplasm.

"We were amazed to find that the cells not only expressed reflectin but also packaged the protein in spheroidal nanostructures and distributed them throughout the cells' bodies," said UCI biomedical engineer Alon Gorodetsky.

Using quantitative phase microscopy, the researchers showed these proteins changed the way light was scattering through the engineered cells, compared to kidney cells without reflectin.

They then exposed the reflectin-expressing cells to different levels of sodium chloride and found they could adjust the levels of light being transmitted through them, as the salt made the reflectin particles swell in size, and change how they arranged themselves.

The more salt, the more light scattered, and the more opaque the cells became. The kidney cells now had tunable light-transmitting and light-reflecting capabilities - essentially an opacity dial of sorts.

Experimental setup. The cells became more opaque after exposure to salt (bottom). (Chatterjee et al, Nat. Commun, 2020)

The reflectin's reaction to salt "bore a superficial resemblance to the acetylcholine-triggered switching of the opacity and broadband reflectance for female D. opalescens squids' leucophore-containing layers", the researchers wrote in their paper.

The team says their success lays the groundwork for incorporating other squid tricks into mammalian cells, like changing colour patterns and iridescence.

It will also allow researchers to further explore the mechanisms behind these abilities, as so far, culturing cephalopod skin cells in a lab has proved very challenging.

Possible future applications could include the ability to image entire living tissues with improved clarity - allowing us to find things that weren't apparent before. The team pointed out how similar studies on jellyfish's green fluorescent proteins led to their now popular use in fluorescence microscopy.

"Our findings may afford a variety of exciting opportunities and possibilities within the fields of biology, materials science, and bioengineering," the team concluded.

This research was published in Nature Communications.

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LED Lighting Horticulture Market Effect and Growth Factors Research and Projection 2020-2027 Estimated by Global Top Players – Cole of Duty

Global LED Lighting Horticulture Market Size, Status and Forecast 2020-2027offers a primary overview of the LED Lighting Horticulture industry coveringDefinition, Classification, Industry Value, Price, Cost and Gross Profit, Share via Region, New Challenge Feasibility Evaluation, Analysis and Guidelines on New mission Investment. LED Lighting Horticulture Market report presents in-intensity insight ofCompany Profile, Capacity, Product Specifications, Production Value, Sales, Revenue, Price, Gross Margin, Market Size and Market Sharesfor topmost prime key vendors( Fluence Bioengineering, Cree, Illumitex, Kessil Lighting, Heliospectra, Hubbell Lighting, LumiGrow, Lemnis Oreon, Osram Sylvania and Smart Grow Technologies.). In the end, there are 4 key segments covered in this LED Lighting Horticulture market report: competitor segment, product type segment, end use/application segment and geography segment.

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Bovine-based Collagen for Biomedical Applications Market: Market Regulation and Competitive landscape Outlook to 2020-2030 – Cole of Duty

Prophecy Market Insights Bovine-based Collagen for Biomedical Applications market research report provides a comprehensive, 360-degree analysis of the targeted market which helps stakeholders to identify the opportunities as well as challenges. The research report study offers keen competitive landscape analysis including key development trends, accurate quantitative and in-depth commentary insights, market dynamics, and key regional development status forecast 2020-2029. It incorporates market evolution study, involving the current scenario, growth rate, and capacity inflation prospects, based on Porters Five Forces and DROT analyses.

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