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A novel highly thermostable xylanase stimulated by Ca2+ from Thermotoga thermarum: cloning, expression and characterization

Posted: February 17, 2013 at 7:00 pm

Background:
Xylanase is an important component of hemicellulase enzyme system. Since it plays an important role in the hydrolysis of hemicellulose into xylooligosaccharides (XOs), high thermostable xylanase has been the focus of much recent attention as powerful enzyme as well as in the field of biomass utilization.
Results:
A xylanase gene (xyn10A) with 3,474 bp was cloned from the extremely thermophilic bacterium Thermotoga thermarum that encodes a protein containing 1,158 amino acid residues. Based on amino acid sequence homology, hydrophobic cluster and three dimensional structure analyses, it was attested that the xylanase belongs to the glycoside hydrolase (GH) families 10 with five carbohydrate binding domains. When the xylanase gene was cloned and expressed in Escherichia coli BL21 (DE3), the specific enzyme activity of xylanase produced by the recombinant strain was up to 145.8 U mg-1. The xylanase was optimally active at 95[degree sign]C, pH 7.0. In addition, it exhibited high thermostability over broad range of pH 4.0-8.5 and temperature 55-90[degree sign]C upon the addition of 5 mM Ca2+. Confirmed by Ion Chromatography System (ICS) analysis, the end products of the hydrolysis of beechwood xylan were xylose, xylobiose, xylotriose, xylotetraose, xylopentaose and xylohexaose.
Conclusions:
The xylanase from T. thermarum is one of the hyperthermophilic xylanases that exhibits high thermostability, and thus, is a suitable candidate for generating XOs from cellulosic materials such as agricultural and forestry residues for the uses as prebiotics and precursors for further preparation of furfural and other chemicals.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/26

Recommendation and review posted by G. Smith

Re-inventing graduate chemistry education?

Posted: February 17, 2013 at 5:11 pm

Recently, some soul searching has been going on in US graduate chemistry education circles.  PhDs have been taking on average six years to finish up, the unemployment level of chemistry graduates has hit record highs of 4.6% and safety standards in university labs have been under the microscope after the tragic death of Sheri Sangji. These problems have been gone over in some detail in a recent report from the American Chemical Society.

The recent symposium on graduate chemistry education at the AAAS meeting went back over these points again, but I was drawn to write about an analysis by the always thought provoking George Whitesides at Harvard.

Whitesides started by making many of the same points found in the ACS report, that US chemical industry jobs had fallen by 300,000 in the past 20 years and that the pharmaceutical industry lost the same number of jobs in the last decade. But he went on to say that chemistry has had a ‘pretty fantastic run’ over the last 50 years pointing to the development of new drugs and materials and new techniques to characterise them.

He also notes that many of these wonderful developments came from industry. Inventions like ROMP, NMR (he acknowledges that this was invented in academia but that industry made it user-friendly), superacids and asymmetric epoxidation. So, if chemistry has been having such a great run, why is there any need to fix anything?

Whitesides says that the Goldman Sachs of the world have now made it impossible for industry to do long-term research of the kind that produced the above advances. Companies need to answer to shareholders simply makes it too difficult to justify expenditure on programmes that aren’t going to deliver a return in the short-term.

This is where he says that universities must step up. They must fill this invention gap that industry has vacated. And graduate students will be an important part of that.

So how do you get graduate students to be more inventive? Whitesides suggests that universities need to teach for breadth and creativity. Universities also need to be unafraid to let old fields die. Quite which fields he has in mind I leave you to guess as he didn’t volunteer any!

Education needs to be reinvented, Whitesides says. He points out that much of what students are taught is archaic and wrong – simplifications that are unhelpful later on. Textbooks are designed to sell, not to help students carry out creative research. Replace them with the web, he suggests, and says this will happen anyway in the next 10 years or so. He also says that grad students need to reduce the extent to which they specialise as 4 years just isn’t enough to get an in depth understanding of an area. At the same time grad students need to prepare to tackle the big problems. If students don’t see what they’re doing is important then why should they value it, and come to that why should anyone else?

He ended by talking about risk. If students don’t take risks and tackle safe problems, that are unimportant and no one cares, then even if they succeed no one takes any notice. If they fail then the consequences are even worse for them. But, if they take on an important problem then it’s a win-win result for them. Even if they don’t succeed they will have provided data that other people are interested in and opened up new avenues to explore.

This all sounds well and good but my question is can everyone really be working on the big problems? So much of scientific discovery has been serendipity. Don’t we need some people looking at the smaller problems, as we never know if they’re more important than we think and feed back into these grand challenges

Patrick Walter

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Source:
http://prospect.rsc.org/blogs/cw/2013/02/17/re-inventing-graduate-chemistry-education/

Recommendation and review posted by G. Smith

Shaking Before/After DBS for Parkinson’s Treatment – Video

Posted: February 17, 2013 at 3:42 pm

http://www.youtube.com/watch?v=a_4_DvquSYQ

Shaking Before/After DBS for Parkinson's Treatment
A man demonstrates the difference in his shaking or tremor before and after DBS Therapy. When medication no longer controls your Parkinson's disease symptoms as well as you would like, talk with your doctor about Medtronic DBS Therapy (deep brain stimulation). See if this FDA-approved therapy can help you return to the life and activities you enjoy. Find out more at http//www.medtronicdbs.com/info. Medtronic DBS Therapy for Parkinson's disease is not for everyone. Not everyone will receive the same results. Talk with your doctor to understand the benefits and risks of deep brain stimulation and determine if Medtronic DBS Therapy is right for you. For further information, please visit http://www.medtronicdbs.com A prescription is required. This video contains information about therapies approved for use in the United States and is intended to be viewed by residents of the United States.

By: MedtronicDBSTherapy<span style="color: #666666; font-size: 11px;"

View post:
Shaking Before/After DBS for Parkinson’s Treatment – Video

Source:
http://www.longevitymedicine.tv/shaking-beforeafter-dbs-for-parkinsons-treatment-video/

Recommendation and review posted by G. Smith

DBS for Parkinson’s Demo: System Off and On – Video

Posted: February 17, 2013 at 3:42 pm

http://www.youtube.com/watch?v=dHcQyj9jdec

DBS for Parkinson's Demo: System Off and On
A man shows how DBS Therapy helps control movement symptoms of Parkinson's disease by temporarily turning off his DBS system. When medication no longer controls your Parkinson's disease symptoms as well as you would like, talk with your doctor about Medtronic DBS Therapy (deep brain stimulation). See if this FDA-approved therapy can help you return to the life and activities you enjoy. Find out more at http//www.medtronicdbs.com/info. Medtronic DBS Therapy for Parkinson's disease is not for everyone. Not everyone will receive the same results. Talk with your doctor to understand the benefits and risks of deep brain stimulation and determine if Medtronic DBS Therapy is right for you. For further information, please visit http://www.medtronicdbs.com A prescription is required. This video contains information about therapies approved for use in the United States and is intended to be viewed by residents of the United States.

By: MedtronicDBSTherapy<span style="color: #666666; font-size: 11px;"

Read the original post:
DBS for Parkinson’s Demo: System Off and On – Video

Source:
http://www.longevitymedicine.tv/dbs-for-parkinsons-demo-system-off-and-on-video/

Recommendation and review posted by G. Smith

Walking Before/After DBS for Parkinson’s Treatment – Video

Posted: February 17, 2013 at 3:42 pm

http://www.youtube.com/watch?v=C3i-0RJ6iFo

Walking Before/After DBS for Parkinson's Treatment
Patient shows how Parkinson's used to affect his walking, versus how he walks while receiving DBS Therapy. When medication no longer controls your Parkinson's disease symptoms as well as you would like, talk with your doctor about Medtronic DBS Therapy (deep brain stimulation). See if this FDA-approved therapy can help you return to the life and activities you enjoy. Find out more at http//www.medtronicdbs.com/info. Medtronic DBS Therapy for Parkinson's disease is not for everyone. Not everyone will receive the same results. Talk with your doctor to understand the benefits and risks of deep brain stimulation and determine if Medtronic DBS Therapy is right for you. For further information, please visit http://www.medtronicdbs.com A prescription is required. This video contains information about therapies approved for use in the United States and is intended to be viewed by residents of the United States.

By: MedtronicDBSTherapy<span style="color: #666666; font-size: 11px;"

Read more from the original source:
Walking Before/After DBS for Parkinson’s Treatment – Video

Source:
http://www.longevitymedicine.tv/walking-beforeafter-dbs-for-parkinsons-treatment-video/

Recommendation and review posted by G. Smith

Capture 2 1 7 2013 8 49 AM – Video

Posted: February 17, 2013 at 3:42 pm


Capture 2 1 7 2013 8 49 AM
This is a video I made of myself off all medication. I have Young onset Parkinson #39;s disease. I got DBS in December of 2011. I made this video so people could see what Parkinson #39;s disease actually looks like. I also made it so you can see the difference in my condition with DBS on and DBS off.

By: Robbin page

Continue reading here:
Capture 2 1 7 2013 8 49 AM - Video

Recommendation and review posted by Fredricko


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