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Category Archives: Genetic Therapy
FDI row: Trinamool may pull out its ministers, NDA steps up protest – Daily News & Analysis
![]() Business Today | FDI row: Trinamool may pull out its ministers, NDA steps up protest Daily News & Analysis Place: New Delhi, Kolkata | Agency: PTI Trinamool Congress, a major UPA constituent, may consider pulling out its ministers from the government in protest against allowing FDI in retail and diesel price hike, and giving it outside support. Mamata may pull out ministers, give outside supportHindustan Times Decision to allow FDI in retail taken under US pressure:CPIBusiness Standard Want Mamata in UPA, but no problem if she leaves: CongressDaily Bhaskar |
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Posted in Genetic Therapy
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Anna Hazare distancing himself from Kejriwal? – India Today
![]() India Today | Anna Hazare distancing himself from Kejriwal? India Today In an apparent sign of distancing himself from the Arvind Kejriwal-led group's political foray, social activist Anna Hazare on Sunday said politics will not deliver a better future for people and asked those planning to join his anti-corruption ... Hazare distancing himself from Kejriwal?Press Trust of India Politics will not bring change: Anna HazareIBNLive.com Politics will not bring change: AnnaBusiness Standard CanIndia News all 31 news articles » |
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Haqqani notification an internal matter of US, says cautious Pak – Times of India
![]() The Hindu | Haqqani notification an internal matter of US, says cautious Pak Times of India ISLAMABAD: In the midst of 'feel good' vibes in Indo-Pak ties, the US decision to designate the Haqqani network as a terror outfit competed for front page space in Pakistani newspapers with Islamabad's careful neutral reaction to the American move ... Pakistan visit fruitful: Krishna (Third Lead)Firstpost Krishna wishes peace for Pak; conveys desire to build trustThe Hindu India desires of peaceful relationship with Pak: KrishnaAll India Radio Livemint -Rediff all 1,612 news articles » |
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Thackerays themselves are ‘infiltrators’ in Maharashtra: Lalu Prasad – Economic Times
![]() Moneycontrol.com | Thackerays themselves are 'infiltrators' in Maharashtra: Lalu Prasad Economic Times "The forefathers of the Thackerays hailed from Bihar and migrated to Mumbai generations ago as has been corroborated by a book written by a late Thackeray family member," Lalu said. PATNA: Hitting out at Shiv Sena patriarch Bal Thackeray and his family ... Thackerays are 'infiltrators' in Mumbai, should leave state immediately: Lalu ...Times of India Won't allow Pak cricketers to play here, says Bal ThackerayDaily News & Analysis Thackerays' same old game only adds to media TRPOneindia The Hindu all 335 news articles » |
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Mumbai: Cartoonist Aseem Trivedi arrested, charged with sedition for ‘mocking … – IBNLive.com
![]() IBNLive.com | Mumbai: Cartoonist Aseem Trivedi arrested, charged with sedition for 'mocking ... IBNLive.com Mumbai: Yet another cartoonist has become the victim of the ongoing policing by political parties. Cartoonist Aseem Trivedi was on Sunday arrested for allegedly posting "ugly and obscene" content on his web portal. Trivedi was arrested in Mumbai and ... Cartoonist Trivedi remanded to police custodyThe Hindu Anna's cartoonist supporter sent to police custodyTimes of India Anti-corruption cartoonist Aseem Trivedi arrested on sedition chargesIndia Today Business Standard -indiablooms all 38 news articles » |
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Evaluation of the immune responses induced by four targeted DNA vaccines encoding the juvenile liver fluke antigen, cathepsin B in a mouse model
Background:
Liver fluke can infect cattle and sheep, and is also emerging as a human pathogen in developing countries. Cathepsin B (Cat B2) is a major cysteine protease secreted by the juvenile flukes. To enhance the immune responses of Cat B2, the cDNA sequence was fused with four different DNA vaccine vectors. The induced cellular and antibody responses were compared in vaccinated mice.
Methods:
The following recombinant DNA vaccine constructs were constructed: empty vector VR1012 as negative control, cytoplasmic construct pVR1012 Cat B2, secretory construct pVR1020 Cat B2, chemokine-fused construct pMCP3 Cat B2 and lymph node targeting construct pCTLA-4 Cat B2. Plasmids were constructed using standard procedures, and positive constructs screened and selected using restriction digestion analysis followed by sequence analysis. The constructs were then tested in Cos - 7 cells for in vitro expression, which was analysed using immunoblotting. Subsequently, female BALB/c mice were immunised with DNA constructs as vaccines. Elicited antibody responses were measured using ELISA. The ratio between IgG1 and IgG2a antibody responses was estimated among different vaccine groups. IgG antibody avidity assay was performed and the relative avidity index was calculated. The induced cytokine production from splenocytes of vaccinated animals was estimated using ELISPOT.
Results:
DNA vaccine constructs carrying Cat B2 were expressed in Cos-7 cell lines and encoded protein was recognised using western blotting using rat anti- cathepsin B antibody. DNA vaccines elicited high Cat B2- specific IgG, IgG1, IgE and also modest IgG2a antibody responses. Cat B2 specific IL-4 T cell responses were also observed in Cat B2 vaccinated mice. The comparison of immunogenic potential in each of these constructs was demonstrated as enhanced antibody responses on the lymph-node targeting vector pCTLA-4 Cat B2, the high antibody avidity of chemo-attractant pMCP3 Cat B2 and stronger T cellular responses of non-secretory DNA vaccine pVR1012 Cat B2 in vaccinated animals.
Conclusion:
This study showed that the targeting DNA vaccine strategies enhanced specific immune responses to juvenile fluke Cat B2. The results of our current study have demonstrated that a gene-based vaccine as an immunotherapeutic approach to combat Fasciola infection may be feasible.Source:
http://www.gvt-journal.com/rss/
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