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Category Archives: Biotechnology
Second Annual BioFORCE Camp Teaches Biotechnology To High School Students 10pm – Video
Second Annual BioFORCE Camp Teaches Biotechnology To High School Students 10pm
By: JennyWalshReporter
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Second Annual BioFORCE Camp Teaches Biotechnology To High School Students 10pm - Video
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B7 Microorganisms and biotechnology – Video
B7 Microorganisms and biotechnology
OCR GCSE Biology B7 Using MOs for biotechnology.
By: NottsAST
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B7 Microorganisms and biotechnology - Video
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Hot Topics in Biotechnology IP Law – Video
Hot Topics in Biotechnology IP Law
Recorded on March 26, 2004. Hot Topics in Intellectual Property Law Symposium. Appearing: Bud Nelson (Chief IP Counsel, Trimeris, Inc.), speaker ; Virginia B...
By: dukelaw
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Hot Topics in Biotechnology IP Law - Video
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Second Annual BioFORCE Camp Teaches Biotechnology To High School Students 6pm – Video
Second Annual BioFORCE Camp Teaches Biotechnology To High School Students 6pm
By: JennyWalshReporter
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Identification of multiple interacting alleles conferring low glycerol and high ethanol yield in Saccharomyces cerevisiae ethanolic fermentation
Background:
Genetic engineering of industrial microorganisms often suffers from undesirable side effects on essential functions. Reverse engineering is an alternative strategy to improve multifactorial traits like low glycerol/high ethanol yield in yeast fermentation. Previous rational engineering of this trait always affected essential functions like growth and stress tolerance. We have screened Saccharomyces cerevisiae biodiversity for specific alleles causing lower glycerol/higher ethanol yield, assuming higher compatibility with normal cellular functionality. Previous work identified ssk1E330N...K356N as causative allele in strain CBS6412, which displayed the lowest glycerol/ethanol ratio.
Results:
We have now identified a unique segregant, 26B, that shows similar low glycerol/high ethanol production as the superior parent, but lacks the ssk1E330N...K356N allele. Using segregants from the backcross of 26B with the inferior parent strain, we applied pooled-segregant whole-genome sequence analysis and identified three minor quantitative trait loci (QTLs) linked to low glycerol/high ethanol production. Within these QTLs, we identified three novel alleles of known regulatory and structural genes of glycerol metabolism, smp1R110Q,P269Q, hot1P107S,H274Y and gpd1L164P as causative genes. All three genes separately caused a significant drop in the glycerol/ethanol production ratio, while gpd1L164P appeared to be epistatically suppressed by other alleles in the superior parent. The order of potency in reducing the glycerol/ethanol ratio of the three alleles was: gpd1L164P > hot1P107S,H274Y >= smp1R110Q,P269Q.
Conclusions:
Our results show that natural yeast strains harbor multiple specific alleles of genes controlling essential functions, that are apparently compatible with survival in the natural environment. These newly identified alleles can be used as gene tools for engineering industrial yeast strains with multiple subtle changes, minimizing the risk of negatively affecting other essential functions. The gene tools act at the transcriptional, regulatory or structural gene level, distributing the impact over multiple targets and thus further minimizing possible side-effects. In addition, the results suggest polygenic analysis of complex traits as a promising new avenue to identify novel components involved in cellular functions, including those important in industrial applications.Source:
http://www.biotechnologyforbiofuels.com/content/6/1/87
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Biotechnology to fight air pollution – futuris – Video
Biotechnology to fight air pollution - futuris
http://www.euronews.com/ Using biotechnology to fight pollution is the challenge for a European Union research project. Under the spotlight - a specific kind...
By: Euronews
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