Teitoku Kakine (A Certain Magical Index) achieved a form of immortality by creating a human tissues (and a new body) out of his Dark Matter.
Ladylee (A Certain Magical Index) is an immortal, in that when she grew weary of living, she sought to use powerful magic to kill her, which did not work.
Tenzen Yakushiji (Basilisk) having his symbiote "eat" away his wounds and restoring any ravages of time or battle, even reattaching his head by sealing the cut.
10 years after Tenzen's death, Joujin (Basilisk) gained the symbiote that was Tenzen's spirit, "eating" away any wounds aging the same way Tenzen's symbiote did.
Skull Knight (Berserk) is the mysterious 1,000 year old enemy of the God Hand and Apostles.
Nosferatu Zodd (Berserk), the 300 year old "God of the Battlefields and Combat".
Wyald (Berserk), the 100 year old leader of the Black Dog Knights.
Behelits (Berserk) are stone fetishes of unknown supernatural origin said to govern the fate of humanity. They are used primarily for summoning the angels of the God Hand, at which point their owners are granted a wish in exchange for a sacrifice.
Creed Diskenth (Black Cat) possesses the God's Breath nano-machines within his body, regenerating even fatal wounds in seconds and maintaining his youth, thus granting him immortality aside from any brain damage being irreparable.
Ssuke Aizen (Bleach) gained immortality after fusing with the Hgyoku.
C.C (Code Geass) is immortal.
V.V (Code Geass) is immortal.
Due to the contradiction caused by the fusion of the absolutely immortal Zamasu and the mortal Goku Black, Merged Zamasu (Dragon Ball) has imperfect immortality.
Zeref (Fairy Tail) was cursed by Ankhseram with his contradiction curse which gives him uncontrollable Death Magic and Immortality.
Mavis Vermilion (Fairy Tail) was cursed with immortality after casting an incomplete Law spell.
Kager (Flame of Recca) using a forbidden spell that opens a time portal, but it traps her outside of space-time, rendering her completely immortal.
The Truth (Fullmetal Alchemist) is invincible, immortal and invulnerable.
Utsuro (Gintama) possesses immortality by harnessing the Altana energy of Earth to prevent aging and recover from wounds and diseases.
Kouka (Gintama) possessed immortality by harnessing the Altana energy of Kouan to prevent aging and recover from wounds and diseases. However, when she left the planet for good, she weakened overtime and died.
China (Hetalia) is the only nation stated to be truly immortal.
Yta (Mermaid Saga) is a 500 years old immortal since unwittingly eating mermaid's flesh.
Mana (Mermaid Saga) is a 15 years old immortal since being fed mermaid's flesh.
Masato (Mermaid Saga) is an 800 years old immortal since eating mermaid's flesh.
Ban, the Undead (Nanatsu no Taizai) acquired immortality after drinking the Fountain of Youth.
Meliodas (Nanatsu no Taizai) was cursed with the immortality by the Demon King.
Orochimaru (Naruto) considers himself immortal with his Living Corpse Reincarnation to transfer his soul to another body and his Cursed Seals as anchors of his conscious.
Hidan's (Naruto) main advantage is his inability to die by physical damage, though he is vulnerable to death by lack of nutrient.
Kakuzu (Naruto) attained a form of immortality (though he denies to think of it as such) by tearing hearts out of others and integrating them into himself, extending his lifespan. He kept five inside him at all times.
Madara Uchiha (Naruto) claims he has achieved complete immortality due to hosting the Shinju, as he regenerated form his torso being blown apart. Only when the tailed beasts were all pulled out of him did he die.
Kaguya tsutsuki (Naruto) is immortal, in that she has tremendous regenerative powers, and that the only way to defeat her is to seal her person away by splitting her chakra into the nine tailed beasts.
Gemma Himuro (Ninja Scroll) putting his severed body parts back together, even his head is possible, rendering him immortal.
Due to her race, Jibril (No Game No Life) has reached 6407 years of age, she also has incredibly vast knowledge and high magical abilities, in two words; she gathers many old and new knowledge, in other words; she can no longer age or die.
Yume Hasegawa (Pupa) is an immortal monster incarnated into human form, possessing regenerative abilities that rendered her very difficult to kill.
Utsutsu Hasegawa (Pupa) has been fed the flesh of her immortal "sister", giving him tremendous regenerative powers that made him more or less immortal.
Rin Asogi (RIN ~Daughters of Mnemosyne~) is immortal, due to a magic spore from Yggdrasil.
Free (Soul Eater) is a werewolf from the Immortal Clan, and therefore, immortal. He can only be harmed and killed by the "Witch-Hunt".
Koj Akatsuki (Strike the Blood) is revealed to be immortal, even by vampire standards after regenerating from complete decapitation.
Tta Konoe (UQ Holder) cannot regrow limbs unless they are completely destroyed, but otherwise is immortal and can reattach any of it, including his head.
Karin Yki (UQ Holder) has one of the highest ranked forms of immortality, stating that she's "not permitted to get hurt or die".
Elder Toguro (Yu Yu Hakusho) stated that his regenerative powers keep him from dying. This prevented him from dying from Kurama's torturous Sinning Tree.
The Pillar Men (JoJo's Bizarre Adventure Part II Battle Tendency)
Dio Brando (JoJo's Bizarre Adventure) become a vampire and gain immortality by using the Stone Mask.
Through the unknown power of his Stand or since merging with DIO's flesh bud, Nijimura's Father (Jojo's Bizarre Adventure Part IV Diamonds Are Unbreakable) is effectively immortal and possess extraordinary healing capabilities.
The Stone Mask (JoJo's Bizarre Adventure Parts I Phantom Blood and II Battle Tendency).
Setsuna F. Seiei (Mobile Suit Gundam 00 The Movie - A wakening of the Trailblazer)
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Recommendation and review posted by G. Smith